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KMID : 0381120150370050441
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Genes and Genomics
2015 Volume.37 No. 5 p.441 ~ p.449
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Characterization of MSTN/GDF11 gene from shrimp Macrobrachium nipponense and its expression profiles during molt cycle and after eyestalk ablation
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Shen Wenying
Ren Gang
Zhu Yaorong
Zhang Xiaodong
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Abstract
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Myostatin (MSTN), also known as the growth differentiation factor-8 (GDF-8), belongs to the transforming growth factor-β superfamily. MSTN is a negative regulator of muscle development in vertebrates. However, the transcriptional regulation of MSTN in freshwater crustaceans is still unclear. In this study, a cDNA encoding for MSTN/GDF11 (Mn-MSTN/GDF11) was cloned from the oriental freshwater shrimp, Macrobrachium nipponense. The full-length cDNA sequence of Mn-MSTN/GDF11 was composed of 1733 nucleotides, including a 5′ UTR of 119 nucleotides, an open read frame of 1359 nucleotides, and a 3′ UTR of 255 nucleotides. The predicted peptide of Mn-MSTN/GDF11 has 452 amino acids with the conserved RXXR cleavage site and nine cysteines. Tissue-specific expression pattern showed that Mn-MSTN/GDF11 is mainly expressed in abdominal muscle. During the course of embryonic development, expression level of Mn-MSTN/GDF11 could be detected after gastrul stage, and reached at the highest level at embryonized-zoea stage. During molt cycle, expression level of Mn-MSTN/GDF11 mRNA was up-regulated significantly at early postmoult stage, but down-regulated gradually in the following molt stages. In the period of 14 days after eyestalk ablation, Mn-MSTN/GDF11 transcripts were significantly decreased in abdominal muscle and heart, but increased in thoracic muscle. The results of this study indicated that Mn-MSTN/GDF11 may play a role in molt cycle and be regulated by hormone secreted in eyestalk.
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KEYWORD
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MSTN/GDF11, Macrobrachium nipponense, Muscle growth, Development stages, Molting, Eyestalk ablation
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